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PROSTATE SPECIFIC ANTIGEN (PSA)
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PSA kit includes all supplies for one test.
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Instructions |
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PSA test instrument |
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Micro tubes and pipettes for mixing |
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Micro tube with dissolution solution |
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Cutting device with cutting mat |
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Built in microtube stand |
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Sterile surgical gloves with sterile
work mat |
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Storage pouch with chain of
custody paperwork |
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| New rape and evidence
collection kit.
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FTA card for DNA
collection included with card with buccal and vagina swab included. |
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Long term storage
for semen sample and buccal sample provided. |
| Call 888-875-7574 for
details. |
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About Prostate Specific Antigen (PSA)
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PSA (Prostate-specific antigen) is a glycoprotein
produced in the prostate and secreted into the seminal fluid. PSA
is one of the major proteins in seminal fluid with concentrations
of 0.2 to 3.0 mg/ml. Its main function is to liquefy the seminal
fluid. This high amount and the fact that PSA is found only at
very low concentrations in female vaginal fluid (0,4-0,9 ng/mL and
0,0-1,25 ng/mL, respectively)2,3 make PSA an interesting marker in
forensic science for the detection of even small amounts of
seminal fluid.
The advantages of a PSA determination are: · The
detection of sperm is possible in cases where no spermatozoa can
be found (for example vasectomized men). · Very small amounts of
sperm can be recorded. Studies of MACALUSO et al. (1999) showed,
that even an amount of only 10 µl sperm increased the PSA
concentration in vaginal fluid ca. 200 fold. · PSA shows a good
stability. In vaginal smear it is detectable in average 27 hours
after the intercourse. Also in 30 years old semen stains PSA could
be recovered at detectable concentrations.1 · PSA is a marker,
which is more specific than the acid phosphatase test. The test
will be affected in its evidence by the fact that other body
fluids as blood or urine can also contain PSA. Whereas the PSA
concentration of male blood serum is normally low (< 4 ng/mL)
and is elevated only in the case of prostatic diseases up to 200
ng/mL, the amount of PSA in urine of healthy men showed in some
cases values of 800 ng/mL (estimated value).
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About the Prostate Specific Antigen (PSA)
cartridge
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(1)
PE-carrier
(2)
Membrane
(3)
monoclonal anti-PSA-antibody
(4)
polyclonal anti-mouse antibody
(5)
Glass fiber pad with gold labeled monoclonal
anti-PSA-antibody
(6)
polyclonal anti-mouse antibody, adjusted to color intensity
of 4 ng PSA/mL
(7)
Adsorbent pad
(8) plastic device
| About the Prostate Specific Antigen (PSA) test
| The membrane contains two monoclonal murine anti-PSA
antibodies as active compounds. One of these antibodies is
immobilized at the test region on the membrane. The
upstream control region and the region of the internal
standard (between control and test region) contain
immobilized polyclonal goat anti-mouse antibodies. The
amount of antibody at the internal standard is adjusted to
a color intensity of the line, which is equal to the color
intensity of the test line at a PSA concentration of 4 ng/mL.
A glass fiber pad downstream of the
membrane is used for sample loading and transmission to a
second fiber pad with the dried and gold labeled second
monclonal murine anti-PSA antibody. |
| PSA at the sample will bind to the re-mobilized
gold-labeled antibody and form a
PSA-gold-labeled-anti-PSA-antibody-complex. Through the
capillary effect of the membrane, the reaction mixture
including the complex is carried upwards with the fluid.
In any case the colored gold labeled anti-PSA-antibody
will bind to the anti-mouse-antibody at the control region
and the region of the internal standard thus developing
two red lines (one at the control region ant one at the
region of the internal standard). These two lines are
independent of the existence of PSA at the sample and
indicate only the correct execution of the test. |
| If the sample contains PSA the PSA-gold-labeled
anti-PSA-antibody complex will bind to the immobilized
monoclonal antibody of the test region that recognizes
another epitope on the PSA molecule. The binding is
indicated by the formation of an additional line. Thus a
PSA positive sample will show three colored lines in the
result well. The line in the middle (internal standard)
correlates with an amount of 4 ng/ml PSA. In some cases it
might be helpful to estimate the amount of PSA in the
sample by comparison of the result line with the internal
standard line. |
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